Overview of Doublet Detecting Softwares¶
Transcription-based doublet detection softwares use the transcriptomic profiles in each cell to predict whether that cell is a singlet or doublet. Most methods simulate doublets by adding the transcriptional profiles of two droplets in your pool together. Therefore, these approaches assume that only a small percentage of the droplets in your dataset are doublets. The table bellow provides a comparison of the different methods.
If you don’t know which demultiplexing software(s) to run, take a look at our Software Selection Recommendations based on your dataset.